Simple Alternatives to Biologics Sample Purification via FPLC
Fast protein liquid chromatography (FPLC) is a common purification technique used widely in various biologics preparation. Often, we complain how slow chromatography columns are, but we have ignored that FPLC itself can cause more trouble than it is worth.
Typical Troubles for Lab-Scale FPLC
FPLC requires extensive preparation, such as having ample amounts of necessary buffers ready for use. You often need to filter your buffers before use even if they look very clean because potential contaminants can reduce your FPLC performance.
FPLC, as a technique, involves a large amount of troubleshooting if bubbles or other problems arise.
If you count setup time, buffer preparation time, and time spent troubleshooting, it may take 1-2 hours.
Pain Points with FPLC
Do you remember how many times you have had to postpone your purification study to the next day because preparation has taken too long to start a run in the late afternoon?
With impending deadlines, project delays can be terrible. Have you ever wanted to purify a few milligrams of antibody at 4 PM, but realized that you must go through the trouble of hooking up a column to the FPLC and preparing it for a run? This hurdle may have caused you or your team member to push the run to the next day. Similarly, you may want to purify a few milligrams of antibody, only to find out that all FPLC is booked or out of service, forcing you to postpone your study. If you do not have FPLC, but you want to purify a few milligrams of antibody, you may have to sacrifice antibody purity by using techniques other than chromatography.
Solution!
Purilogics has a solution for you! We offer both mini and midi Protein A columns which can be used with only a syringe, instead of the more intricate process of the FPLC (although FPLC compatible). These columns can purify 0.5-4 mg antibody per unit per cycle easily. You do not need to worry about setting up FPLC , ensuring stability of the UV curve, or breaking equipment! It is very simple to use. The fundamental principal of these columns is that they are based on Purilogics Purexa™ PrA membrane technology. These columns maintain high binding capacity at very high flowrates.
If you are interested in learning more, please feel free to contact us at bd@purilogics.com or +1(864) 455-1457.
