Purexa™ PrA mini - Fast Antibody Purification within 1 Minute

Here we are providing an antibody purification solution for numerous scenarios:

You need 1-2 mg purified antibody immediately and your lab FPLC or centrifuge is occupied or unavailable;
You do not want the hassle of using magnetic beads, FPLC, or centrifugation;
You have a lot of samples to purify and the FPLC is too slow to get the job done quickly;
You need highly concentrated antibody solution;
You want to get your purification completed in minutes;
….

Purexa™ PrA mini is designed for rapid processing of small quantity of antibodies, especially at low titers. The purification can be completed using syringes. In an example, 14 mL antibody solution at 0.15 mg/mL was injected onto the Purexa PrA mini using a syringe, followed by 2 mL washing and 1-1.5 mL elution. The whole purification process including equilibration, loading, washing and elution takes shorter than 1 minute! During elution, the first 0.5 mL was discarded.

As is shown in the figures, a majority of the antibody was eluted in the second 0.5 mL elution fraction, which had a concentration of 3.3 mg/mL and was corresponding to a recovery of 78%. Another 13% can be recovered in the third 0.5 mL elution fraction.